First, the class divided up into several groups,then each group was required to find a baseline. The baseline is the index of the initial concentration of H2O2 in the solution. Before the groups could determine the amount of H2O2 used up in the later experiment, it was necessary for each to find a baseline. There is data for six baselines, therefore six groups, but only five of those six group's baseline's were used for the time course determination experiment.
The average baseline of these six groups was 3.76. After the baselines were determined then five of the six groups were assigned a different section of the time-course determination experiment. In this experiment, the groups were to determine the rate at which a 1.5% H2O2 solution decomposes when catalyzed by the purified catalase extract. Groups were assigned To 10, 30, 60, 120, and 180 second intervals. The data as shown is for the respected times as they appear on the spread sheet.
As the graph of the data shows, the amount of H2O2 used continued to rise for the extended lengths of time. For the time intervals, the amount of H202 used showed little signs of leveling off, but does show a nice steady rise as the time periods increased. The reason the curve rises is simply because for more time, more H202 is consumed in the reaction. (See Graph Below).
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