b)Chemicals: catalase, KMnO4, water, H2O2, liver
c)Safety Features: goggles, gloves
PROCEDURE
Exercise 1
PURPOSE: To determine the amount of H2O2 initially present in a 1.5% solution.
1. Put 10 ml of a 1.5% H2O2 solution in a beaker.
2. Add 1ml of H2O to this solution.
3. Add 10 ml H2SO4.
4. Mix well.
5. Remove 5 ml and assay for the amount of H2O2 present. Use a burrett to add KMnO4a drop at a time until a persistent pink or brown color is obtained. Remember to mix the solution well. Record data!
Exercise 2
PURPOSE: To determine the rate of spontaneous conversion of H2O2 to H2O and O2 in an uncatalyzed reaction.
1. Transfer 15 ml of a 1.5% H2O2 solution to a 50ml glass beaker.
2. Store this solution uncovered for approximately 24 hours at room temperature.
Exercise 3
PURPOSE: To determine the rate at which a 1.5% H2O2 solution decomposes when catalyzed by the purified catalase extract.
1. Test reaction in Exercise 1 for ten seconds. Record data.
2. Test reaction in Exercise 1 for thirty seconds. Record data.
3. Repeat this process for time intervals of 60, 120, and 180 seconds and record data.
GO to your HOME!!!
Exercise 2
1. Transfer 15 ml of a 1.5% H2O2 solution to a 50ml glass beaker.
2. Store this solution uncovered for approximately 24 hours at room temperature.